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TL-988 Series

Real-time Quantitative PCR Systems

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n       Real-time Quantitative PCR Fluorescence Detecting System

The TL988 System provides an efficiency and high-speed solution for PCR analysis. It delivers increased sensitivity and a wider dynamic range more rapidly than traditional PCR.  QPCR gives you a choice of quantitative methods for gene expression. The TL988 is very useful and important for life science research and curative evaluation, and it is innovative and pioneering products for the prevention, diagnosis, evaluation and monitoring of diseases.

 

n       Applications

¡§         Quantitative PCR detection in science research.

¡§         Real-time quantitative PCR detection for clinical diagnoses, such as the infectious disease, tumor, genetic disease.

¡§         Quantitative DNA/RNA detection of virus, such as HBV, venereal disease, etc.

 

n       Features

 

 n        Working principle

                        

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 n        Heating & cooling unit

Applying the advantages of thermoelectric (TE, Peltier Effect) module makes temperature change rapidly and uniform. The temperature ramp rate is 3.0 ¡æ/sec. This ramping speed will allow you to complete a standard 40 cycles, two-step QPCR reaction in about 45 minutes to 70 minutes (based on reagents). Hot-lid design permits oil-free operation.

 

 n        Optical unit

¡§         An array of LED collimates to corresponding wells. High-light and long-lived LED excitation light source does not need maintenance or preheating.

¡§         The sensitive photomultiplier tube PMT offers high sensitivity, broad dynamic range and low crosstalk.

¡§         An array of detecting coaxial optical fibers focus on each well¡¯s bottom ensure minimal light loss.

¡§         Special monochromator filter reduces crosstalk and light scattering.

¡§         The wavelength of fluorescence detection can be changed in term of customer¡¯s requirement.

 

 n        Operation system and Application software

¡§         Computer¡¯s operating system environment is MS Window 2000/XP. Application software interface is friendly windows style, menu option, easily operating.

¡§         Monitoring real-time amplification plots and data analysis during a PCR run.

¡§         Supply software package of medical case and database management and plot display.

 

 n        Quantification

After quantitative analysis for the standard sample, the instrument will get a standard curve of Ct value related to copy number. Comparing the Ct value of unknown sample with standard curve, the instrument can calculate the original copy number of unknown sample by quantitative analysis. TL988 has a wide linear detecting range, which can detect 101-1010 DNA copies/ml.

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n       Specifications

 

 

Reagents

FAM, FITC, SYBR, various fluorescence PCR reagents, such as TaqMan probe, etc

Sample capacity

48 well block; 96 well block; 36 well block

Sample handing

Thick-walled 0.2 ml PCR tubes

Sample volume

20-100 µl

Wavelength of exciting fluorescence

470 nm

Wavelength of fluorescence detection

530 nm (changeable)

Monochromator device

Filter, made in USA

Photo detector

PMT (Photomultiplier tube), made in Japan

Heating/cooling devices

TE Peltier (Thermoelectric Peltier Effect module), made in Japan

Speed of ramping of heating

Up to 3 ¡æ/sec

Speed of ramping of cooling

Up to 2 ¡æ/sec

Cycling control

 

Profiles

1-9

Repetitions

1-99

Steps

1-9

Set times

1-9999 sec

Profile linkage

Yes

Thermal range

4.0 ¡æ-99.0 ¡æ

Thermal accuracy

¡À0.5 ¡æ

Thermal uniformity

¡À0.5 ¡æ

Standard curve efficiency

99.7%

Dynamic Range

101-1010 Copies

System interfaces

RS-232C serial interface, bi-directional

Computer operating system

Windows 2000/XP/NT

Input power

AC220¡À22V£¬50¡À1Hz ; 110V(option)

Power

< 800 W

Operating environment

Ambient Temperature 5¡æ-40¡æ

Relative Humidity 30%-70%

Weight

15 kg

Size (W¡ÁD¡ÁH)

500 mm ¡Á 400 mm ¡Á 350 mm

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